Trovafloxacin mesylate is a fluoroquinolone antibiotic widely used in microbiological and pharmacological research. Also known as PF-00345571, this compound is identified under CAS 147059-75-4 and has been studied for its broad-spectrum antibacterial activity. Trovafloxacin mesylate Pfizer development programs contributed to its recognition as a reference compound for investigating bacterial DNA replication and antibiotic resistance mechanisms.
Researchers often analyze Trovafloxacin mesylate chemical structure to better understand its interaction with bacterial enzymes such as DNA gyrase and topoisomerase IV. In addition, Trovafloxacin mesylate molecular weight is an important parameter when preparing solutions and designing reproducible assays across different experimental systems.
Application of Trovafloxacin mesylate
Application Trovafloxacin mesylate is primarily focused on studies involving antibacterial activity and resistance mechanisms. It is commonly used in research exploring how fluoroquinolone antibiotics affect bacterial replication and survival.
- evaluation of antibacterial efficacy against gram-positive and gram-negative bacteria
- investigation of resistance mechanisms linked to DNA gyrase inhibition
- screening of novel antimicrobial compounds in comparative studies
These applications make PF-00345571 a valuable tool for both basic microbiology research and drug discovery programs targeting infectious diseases.
In Vitro
In vitro, Trovafloxacin mesylate is frequently used in bacterial culture assays to assess inhibitory effects on DNA replication. It allows researchers to measure growth suppression, minimum inhibitory concentrations, and bacterial response under controlled conditions.
Such studies provide insight into compound potency, spectrum of activity, and interaction with bacterial targets, forming a basis for further pharmacological evaluation.
In Vivo
In vivo studies of Trovafloxacin mesylate have explored its antibacterial activity in animal infection models, where it demonstrates the ability to reduce bacterial load and improve survival outcomes. These studies are useful for understanding pharmacokinetics, tissue distribution, and systemic efficacy.
Although its clinical use has been limited, it remains relevant in research settings as a model compound for fluoroquinolone activity.
Biochemical and Physiological Actions
The primary biochemical action of Trovafloxacin mesylate involves inhibition of bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and cell division. By blocking these enzymes, the compound disrupts bacterial growth and leads to cell death.
From a physiological perspective, this mechanism is critical for studying antimicrobial action and resistance development, particularly in pathogens with evolving drug resistance profiles.
Features and benefits of Trovafloxacin mesylate
Trovafloxacin mesylate offers a well-characterized profile for antibacterial research, making it suitable for a wide range of experimental applications. When supplied as EBC-98026, it is supported by quality control and analytical validation to ensure reliability.
- broad-spectrum antibacterial activity for diverse research models
- useful reference compound for studying fluoroquinolone mechanisms
- consistent quality supported by validated analytical data
As part of a broader portfolio of bioactive molecules, Trovafloxacin mesylate supports advanced research into antimicrobial mechanisms and helps researchers refine strategies for studying bacterial resistance and drug efficacy.
